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Abstract: By acid-catalyzed reaction of substituted isatins with Girard?s reagent T, new water-soluble isatin-3-acylhydrazones were obtained with high yields. Their antimicrobial activity was evaluated. Selective activity against Gram-positive bacteria (S. aureus 209p and B. cereus 8035) and yeast-like fungus Candida albicans 855?653 along with low hematoxicity was established.

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The present invention relates to specific acylhydrazone compounds, their use as oxidation catalysts and to a process for removing stains and soil on textiles and hard surfaces. The compounds are substituted with a specific cyclic ammonium group adjacent to the acyl group. Further aspects of the invention are compositions or formulations comprising such compounds.

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We report the first examples of purely organic donor?acceptor materials with integrated pi-bowls (piBs) that combine not only crystallinity and high surface areas but also exhibit tunable electronic properties, resulting in a four-orders-of-magnitude conductivity enhancement in comparison with the parent framework. In addition to the first report of alkyne?azide cycloaddition utilized for corannulene immobilization in the solid state, we also probed the charge transfer rate within the Marcus theory as a function of mutual piB orientation for the first time, as well as shed light on the density of states near the Fermi edge. These studies could foreshadow new avenues for piB utilization for the development of optoelectronic devices or a route for highly efficient porous electrodes.

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Metal catalyst and ligand design,
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A hairy cationic nanocellulose (CNCC) was prepared by a two-step reaction. First dialdehyde modified cellulose (DAMC) were prepared by periodate oxidation of cellulose fibers and subsequently DAMC fibers were cationized by a reaction between the aldehyde groups of cellulose and (2-hydrazinyl-2-oxoethyl)-trimethylazanium chloride [Girard?s reagent T (GT)] to produce cationic dialdehyde cellulose (CDAMC) fibers. Subjecting the suspension of CDAMC fibers to a hot-water treatment at 60 C, resulted in the formation of cationic rod-like nanocellulose (CNCC). Atomic force microscopy and transmission electron microscopy showed the CNCC has a width of 5 nm and a length about 120 nm. Zeta potential measurement, Fourier transform infrared spectroscopy, solid carbon-13 NMR and X-ray photoelectron spectroscopy confirmed the presence of cationic groups on CNCC. The positive charge content of CNCC is about 1.68 mmol/g measured by conductometric titration. CNCC has a crystalline index of 67 % and possesses cellulose I crystalline structure.

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The success of applying the tethering strategy in a synthetic molecular system strongly depends on the experimental conditions and is related to the strength of the noncovalent interaction and the competition between the ‘captured’ and unbound ligand for the recognition site. The Royal Society of Chemistry.

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Metal catalyst and ligand design,
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Chemical ionization of organic compounds with negligible vapor pressures (VP) is achieved at atmospheric pressure when the proximal sample is exposed to corona discharge. The vapor-phase analyte is produced through a reactive olfaction process, which is determined to include electrostatic charge induction in the proximal condensed-phase sample, resulting in the liberation of free particles. With no requirement for physical contact, a new contained nano-atmospheric pressure chemical ionization (nAPCI) source was developed that allowed direct mass spectrometry analysis of complex mixtures at a sample consumption rate less than nmol/min. The contained nAPCI source was applied to analyze a wide range of samples including the detection of 1 ng/mL cocaine in serum and 200 pg/mL caffeine in raw urine, as well as the differentiation of chemical composition of perfumes and beverages. Polar (e.g., carminic acid; estimated VP 5.1 × 10-25 kPa) and nonpolar (e.g., vitamin D2; VP 8.5 × 10-11 kPa) compounds were successfully ionized by the contained nAPCI ion source under ambient conditions, with the corresponding ion types of 78 other organic compounds characterized.

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Capillary electrophoresis?mass spectrometry was applied for the analysis of oligosaccharides and N-linked glycans with an attached charge label facilitating electrophoretic migration and electrospray ionization efficiency. Several different labeling strategies have been tested with different tags and tagging reactions including reductive amination and hydrazone formation. However, a formation of multiple labeled N-linked glycans was observed by CE-MS in a positive ion mode when positively charged labels such as aliphatic amines containing a quaternary ammonium group were attached to N-linked glycans by reductive amination. A reaction mechanism explaining a side reaction occurring during the labeling and the multiple product formation was proposed and confirmed by using isotopically labeled N-acetylglucosamine. Finally, it was confirmed that derivatization of sugars via a hydrazone formation can be a simpler method with a high reaction yield suitable for high sensitive CE-ESI/MS analyses of N-linked glycans.

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Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate driven process involving the competition of enzymes that extend the nascent chain. The large diversity of structures, the variations in polarity of the individual saccharide residues, and the poor ionization efficiencies of glycans all conspire to make the analysis arguably much more difficult than any other biopolymer. Furthermore, the large number of glycoforms associated with a specific protein site makes it more difficult to characterize than any post-translational modification. Nonetheless, there have been significant progress, and advanced separation and mass spectrometry methods have been at its center and the main reason for the progress. While glycomic and glycoproteomic analyses are still typically available only through highly specialized laboratories, new software and workflow is making it more accessible. This review focuses on the role of mass spectrometry and separation methods in advancing glycomic and glycoproteomic analyses. It describes the current state of the field and progress toward making it more available to the larger scientific community.

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Leidenfrost levitated droplets can be used to accelerate chemical reactions in processes that appear similar to reaction acceleration in charged microdroplets produced by electrospray ionization. Reaction acceleration in Leidenfrost droplets is demonstrated for a base-catalyzed Claisen?Schmidt condensation, hydrazone formation from precharged and neutral ketones, and for the Katritzky pyrylium into pyridinium conversion under various reaction conditions. Comparisons with bulk reactions gave intermediate acceleration factors (2?50). By keeping the volume of the Leidenfrost droplets constant, it was shown that interfacial effects contribute to acceleration; this was confirmed by decreased reaction rates in the presence of a surfactant. The ability to multiplex Leidenfrost microreactors, to extract product into an immiscible solvent during reaction, and to use Leidenfrost droplets as reaction vessels to synthesize milligram quantities of product is also demonstrated.

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13C-isotope labelling is presented as a novel tool for the study of complex chemical systems. 13C-isotope labelling permits the quantification of all 26 members of a dynamic library from a single 13C NMR spectrum without the need for advanced instrumentation or sophisticated experimental protocols.

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Metal catalyst and ligand design,
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