Category: 494-52-0

So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Wang, Xiaoyu; Qin, Yaqiong; Nie, Cong; Guo, Junwei; Pan, Lining; Xie, Fuwei; Wang, Sheng; Wang, Bing; Zhao, Xiaodong; Wang, Baolin; Jia, Guotao researched the compound: (S)-3-(Piperidin-2-yl)pyridine( cas:494-52-0 ).Related Products of 494-52-0.They published the article 《Smokeless tobacco analysis: Simultaneous extraction and purification of alkaloids, volatile N-nitrosamines, and polycyclic hydrocarbons for GC-MS/MS》 about this compound( cas:494-52-0 ) in Journal of Separation Science. Keywords: smokeless tobacco alkaloid nitrosamine polycyclic hydrocarbon; Alkaloids; Polycyclic aromatic hydrocarbons; Simultaneous extraction; Smokeless tobacco product; Volatile nitrosoamines. We’ll tell you more about this compound (cas:494-52-0).

Several smokeless tobacco products are available in the market and comprise complex chem. matrixes. Sample preparation for anal. of the multiple classes of harmful compounds in smokeless tobacco products is highly cumbersome. In this study, a simultaneous extraction scheme was developed for three toxic analyte classes in smokeless tobacco products using a two-phase solution consisting of 5% aqueous NaOH and dichloromethane in a 1:4 ratio. The dichloromethane extract was used to analyze four alkaloids directly at levels greater than ppm; however, passing the layer through a silica cartridge for further purification and concentration was necessary for determining 18 polycyclic aromatic hydrocarbons and four volatile N-nitrosoamines at the ppt level. The multitargets were determined by using gas chromatog. with tandem mass spectrometry. The limits of detection for the 18 polycyclic aromatic hydrocarbons, four volatile N-nitrosoamines, three minor alkaloids, and nicotine were 0.2-1.2, 0.2-0.4, 0.6-1.0, and 10.2μg/g, resp. Four different smokeless tobacco substrates were fortified with three levels of mixed standards, and the recoveries ranged between 83 and 110%. The method was highly efficient, reduced the sample amounts, solvents, and the time required by approx. 60%. The method was used to assay 18 smokeless tobacco products, and showed potentials in assaying drugs and other plant-based substrates.

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Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis》. Authors are Boyeldieu, Anne; Ali Chaouche, Amine; Ba, Moly; Honore, Flora Ambre; Mejean, Vincent; Jourlin-Castelli, Cecile.The article about the compound:(S)-3-(Piperidin-2-yl)pyridinecas:494-52-0,SMILESS:C1(C=NC=CC=1)[C@@H]1CCCCN1).Synthetic Route of C10H14N2. Through the article, more information about this compound (cas:494-52-0) is conveyed.

Abstract: The core of the chemotaxis system of Shewanella oneidensis is made of the CheA3 kinase and the CheY3 regulator. When appropriated, CheA3 phosphorylates CheY3, which, in turn, binds to the rotor of the flagellum to modify the swimming direction. In this study, we showed that phosphorylated CheY3 (CheY3-P) also plays an essential role during biogenesis of the solid-surface-associated biofilm (SSA-biofilm). Indeed, in a ΔcheY3 strain, the formation of this biofilm is abolished. Using the phospho-mimetic CheY3D56E mutant, we showed that CheY-P is required throughout the biogenesis of the biofilm but CheY3 phosphorylation is independent of CheA3 during this process. We have recently found that CheY3 interacts with two diguanylate cyclases (DGCs) and with MxdA, the c-di-GMP effector, probably triggering exopolysaccharide synthesis by the Mxd machinery. Here, we discovered two addnl. DGCs involved in SSA-biofilm development and showed that one of them interacts with CheY3. We therefore propose that CheY3-P acts together with DGCs to control SSA-biofilm formation. Interestingly, two orthologous CheY regulators complement the biofilm defect of a ΔcheY3 strain, supporting the idea that biofilm formation could involve CheY regulators in other bacteria.

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Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《A Simple and High-Throughput LC-MS-MS Method for Simultaneous Measurement of Nicotine, Cotinine, 3-OH Cotinine, Nornicotine and Anabasine in Urine and Its Application in the General Korean Population.》. Authors are Oh, Jongwon; Park, Min-Seung; Chun, Mi-Ryung; Hwang, Jung Hye; Lee, Jin-Young; Jee, Jae Hwan; Lee, Soo-Youn.The article about the compound:(S)-3-(Piperidin-2-yl)pyridinecas:494-52-0,SMILESS:C1(C=NC=CC=1)[C@@H]1CCCCN1).Application In Synthesis of (S)-3-(Piperidin-2-yl)pyridine. Through the article, more information about this compound (cas:494-52-0) is conveyed.

Measuring nicotine metabolites is the most objective method for identifying smoke exposure. Liquid chromatography–tandem mass spectrometry (LC-MS-MS) can measure multiple metabolites and is sensitive enough to detect low concentrations of metabolites. Therefore, we developed a simple and high-throughput method for measuring nicotine, cotinine, trans-3′-hydroxycotinine (3-OH cotinine), nornicotine and anabasine for population-based studies using LC-MS-MS. Each 30 µL of urine sample was diluted with 90 µL of acetonitrile containing five deuterated internal standards. Chromatographic separation used a C18 column, and LC-MS-MS analysis was performed with a multiple reaction monitoring mode. The chromatographic run time for each sample was 6.5 min. The method was validated by evaluating selectivity, interference, limit of detection, lower limit of quantification, precision, accuracy, linearity, extraction recovery, matrix effect and carryover according to guidelines. Our methods required a short preparation time (∼20 min) while simultaneously measuring five markers for smoking status. No endogenous or exogenous interference was found. Our method showed excellent precision and accuracy: within-run coefficient of variation (CV) 2.9-9.4%, between-run CV 4.8-8.7% and bias -10.1 to 5.3%. Linear dynamic ranges were 1-10,000 ng/mL for nicotine, nornicotine and anabasine; 2-5,000 ng/mL for cotinine and 5-15,000 ng/mL for 3-OH cotinine. Extraction recovery was consistent (87-109%) across concentrations. No significant matrix effect or carryover was observed. The validated method was applied to 849 urine samples. In samples from the 125 current smokers, nicotine, cotinine, 3-OH cotinine, nornicotine and anabasine were detected in 97.6, 99.2, 98.4, 96.8 and 87.2%, respectively. No markers were detected in 93.9% of 609 nonsmokers. The overlapping detection of multiple markers made it possible to identify the smoking status even in current smokers with a low concentration of cotinine. Our LC-MS-MS method using a simple sample preparation technique is sensitive and effective for screening of smoking status in the general population.

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Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

Name: (S)-3-(Piperidin-2-yl)pyridine. Aromatic compounds can be divided into two categories: single heterocycles and fused heterocycles. Compound: (S)-3-(Piperidin-2-yl)pyridine, is researched, Molecular C10H14N2, CAS is 494-52-0, about A rapid LC-MS/MS method for simultaneous determination of nicotine and its key derivatives including hydroxylation isomers. Author is Shen, Yixiao; Zhang, Ning; Prinyawiwatkul, Witoon; Xu, Zhimin.

A rapid high pressure liquid chromatog. coupled with tandem mass spectrometry method (LC-MS/MS) was developed for simultaneously determining nicotine (NT) and its derivatives including cotinine (CT), trans-3-hydroxycotinine (HC), nornicotine (NNT), anatabine (AT), anabasine (AB) and amino ketone (4-(methylamino)-1-(3-pyridyl)1-butanone) (AK) as well as two important metabolites isomers 2-hydroxynicotine (2-HN) and 6-hydroxynicotine (6-HN) in tobacco leaves. Different from other methods, 2-HN and 6-HN isomers can be successfully separated and quantified through a combination of amide and C18 columns and optimized multiple reaction monitoring mode of tandem mass spectrometry. The limits of detections (LOD) and quantification (LOQ) of these compounds were 0.02 and 0.06 ng/mL for CT and HC, 0.05 and 0.15 ng/mL for AK and 6-HN, and 0.1 and 0.3 ng/mL for AB, NNT, AT, 2-HN and NT, resp. Their recoveries ranged from 86.1% for 2-HN to 102.2% for AK by using solvent extraction assisted with ultrasonication. The developed method was validated through determination of the compounds in different regular and genetically modified (for lowering nicotine) tobacco leaves. Conclusively, it is a robust and rapid method for simultaneous quantification of nicotine and its derivatives in tobacco products. The protocol of the method could also be applied in developing anal. method for tracking the toxic substances synthesis and metabolism in tobacco leaves or other herbal plants.

There is still a lot of research devoted to this compound(SMILES：C1(C=NC=CC=1)[C@@H]1CCCCN1)Name: (S)-3-(Piperidin-2-yl)pyridine, and with the development of science, more effects of this compound(494-52-0) can be discovered.

Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic.Ren, Mengjuan; Zhang, Mengyue; Yang, Huijuan; Shi, Hongzhi researched the compound: (S)-3-(Piperidin-2-yl)pyridine( cas:494-52-0 ).Reference of (S)-3-(Piperidin-2-yl)pyridine.They published the article 《Reducing the nicotine content of tobacco by grafting with eggplant》 about this compound( cas:494-52-0 ) in BMC Plant Biology. Keywords: tobacco eggplant grafting nicotine; Differentially expressed genes; Grafting; Nicotine content; Tobacco; Transcription factors. We’ll tell you more about this compound (cas:494-52-0).

Background: Nicotine is a stimulant and potent parasympathomimetic alkaloid that accounts for 96-98% of alkaloid content. A reduction in the amount of nicotine in cigarettes to achieve a non-addictive level is necessary. We investigated whether replacing tobacco root with eggplant by grafting can restrict nicotine biosynthesis and produce tobacco leaves with ultra-low nicotine content, and analyzed the gene expression differences induced by eggplant grafting. Results: The nicotine levels of grafted tobacco leaves decreased dramatically. The contents of nornicotine, anabasine, NNN, NNK, NAT, total TSNAs and the nicotine of mainstream cigarette smoke decreased, and the contents of amino acids and the precursors of alkaloids increased in grafted tobacco. Eggplant grafting resulted in the differential expression of 440 genes. LOC107774053 had higher degrees in two PPI networks, which were regulated by LOC107802531 and LOC107828746 in the TF-target network. Conclusions: Replacing tobacco root with eggplant by grafting can restrict nicotine biosynthesis and produce tobacco leaves with ultra-low or zero nicotine content. The differential expression of LOC107774053 may be associated with eggplant grafting.

If you want to learn more about this compound((S)-3-(Piperidin-2-yl)pyridine)Reference of (S)-3-(Piperidin-2-yl)pyridine, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(494-52-0).

Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《The effect of toxic pyridine-alkaloid secondary metabolites on the sunbird gut microbiome》. Authors are Gunasekaran, Mohanraj; Lalzar, Maya; Sharaby, Yehonatan; Izhaki, Ido; Halpern, Malka.The article about the compound:(S)-3-(Piperidin-2-yl)pyridinecas:494-52-0,SMILESS:C1(C=NC=CC=1)[C@@H]1CCCCN1).COA of Formula: C10H14N2. Through the article, more information about this compound (cas:494-52-0) is conveyed.

Abstract: Sunbirds feed on tobacco tree nectar which contains toxic nicotine and anabasine secondary metabolites. Our aim was to understand the effect of nicotine and anabasine on the gut microbiota composition of sunbirds. Sixteen captive sunbirds were randomly assigned to two diets: artificial nectar either with (treatment) or without (control) added nicotine and anabasine. Excreta were collected at 0, 2, 4 and 7 wk of treatment and samples were processed for bacterial culture and high-throughput amplicon sequencing of the 16S rRNA gene. The gut microbiome diversity of the treated and control birds changed differently along the seven-week experiment While the diversity decreased in the control group along the first three samplings (0, 2 and 4 wk), it increased in the treatment group. The microbiota composition analyses demonstrated that a diet with nicotine and anabasine, significantly changed the birds′ gut microbiota composition compared to the control birds. The abundance of nicotine- and anabasine- degrading bacteria in the excreta of the treated birds, was significantly higher after four and seven weeks compared to the control group. Furthermore, anal. of culturable isolates, including Lactococcus, showed that sunbirds′ gut-associated bacteria were capable of degrading nicotine and anabasine, consistent with their hypothesised role as detoxifying and nutritional symbionts.

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Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

Aguirre, Luis A.; Davis, Julie K.; Stevenson, Philip C.; Adler, Lynn S. published an article about the compound: (S)-3-(Piperidin-2-yl)pyridine( cas:494-52-0,SMILESS:C1(C=NC=CC=1)[C@@H]1CCCCN1 ).COA of Formula: C10H14N2. Aromatic heterocyclic compounds can be classified according to the number of heteroatoms or the size of the ring. The authors also want to convey more information about this compound (cas:494-52-0) through the article.

Herbivory can induce chem. changes throughout plant tissues including flowers, which could affect pollinator-pathogen interactions. Pollen is highly defended compared to nectar, but no study has examined whether herbivory affects pollen chem. We assessed the effects of leaf herbivory on nectar and pollen alkaloids in Nicotiana tabacum, and how herbivory-induced changes in nectar and pollen affect pollinator-pathogen interactions. We damaged leaves of Nicotiana tabacum using the specialist herbivore Manduca sexta and compared nicotine and anabasine concentrations in nectar and pollen. We then pooled nectar and pollen by collection periods (within and after one month of flowering), fed them in sep. experiments to bumble bees (Bombus impatiens) infected with the gut pathogen Crithidia bombi, and assessed infections after seven days. We did not detect alkaloids in nectar, and leaf damage did not alter the effect of nectar on Crithidia counts. In pollen, herbivory induced higher concentrations of anabasine but not nicotine, and alkaloid concentrations rose and then fell as a function of days since flowering. Bees fed pollen from damaged plants had Crithidia counts 15 times higher than bees fed pollen from undamaged plants, but only when pollen was collected after one month of flowering, indicating that both damage and time since flowering affected interaction outcomes. Within undamaged treatments, bees fed late-collected pollen had Crithidia counts 10 times lower than bees fed early-collected pollen, also indicating the importance of time since flowering. Our results emphasize the role of herbivores in shaping pollen chem., with consequences for interactions between pollinators and their pathogens.

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Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: (S)-3-(Piperidin-2-yl)pyridine(SMILESS: C1(C=NC=CC=1)[C@@H]1CCCCN1,cas:494-52-0) is researched.Synthetic Route of C24H40N4O4Rh2. The article 《EAT-18 is an essential auxiliary protein interacting with the non-alpha nAChR subunit EAT-2 to form a functional receptor》 in relation to this compound, is published in PLoS Pathogens. Let’s take a look at the latest research on this compound (cas:494-52-0).

Nematode parasites infect approx. 1.5 billion people globally and are a significant public health concern. There is an accepted need for new, more effective anthelmintic drugs. Nicotinic acetylcholine receptors on parasite nerve and somatic muscle are targets of the cholinomimetic anthelmintics, while glutamate-gated chloride channels in the pharynx of the nematode are affected by the avermectins. Here we describe a novel nicotinic acetylcholine receptor on the nematode pharynx that is a potential new drug target. This homomeric receptor is comprised of five non-α EAT-2 subunits and is not sensitive to existing cholinomimetic anthelmintics. We found that EAT-18, a novel auxiliary subunit protein, is essential for functional expression of the receptor. EAT-18 directly interacts with the mature receptor, and different homologs alter the pharmacol. properties. Thus we have described not only a novel potential drug target but also a new type of obligate auxiliary protein for nAChRs.

If you want to learn more about this compound((S)-3-(Piperidin-2-yl)pyridine)Application In Synthesis of (S)-3-(Piperidin-2-yl)pyridine, you may wish to communicate with the author of the article,or consult the relevant literature related to this compound(494-52-0).

Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI

Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 494-52-0, is researched, Molecular C10H14N2, about The use of HPLC-PDA in determining nicotine and nicotine-related alkaloids from E-liquids: a comparison of five E-liquid brands purchased locally, the main research direction is nicotine alkaloid high performance liquid chromatog photodiode array detection; E-liquids; ECIGs; HPLC; aerosol; cigarettes; nicotine; nicotine-related alkaloids; smoke.COA of Formula: C10H14N2.

E-liquid manufacturers are under scrutiny concerning the purity and concentration accuracy of nicotine and the minor nicotine-related alkaloids (NRAs) packaged in their products. In this communication we report concentrations of nicotine and five NRAs (nornicotine, cotinine, anabasine, anatabine, myosmine) from locally purchased E-liquids Methods: Five brands of E-liquids (three bottles each) were purchased locally. Addnl., three bottles of reference E-liquid were prepared Concentrations of nicotine and NRAs from each bottle were measured by HPLC. Concentrations of these alkaloids were also determined from electronic cigarette-generated aerosol and traditional cigarette smoke. Results: Nicotine concentrations in E-liquid brands 1, 2, 3, 4, 5 and in the reference E-liquid were 17.8 ± 4.1, 23.2 ± 0.7, 24.0 ± 0.9, 24.9 ± 0.2, 19.7 ± 0.3 and 20.4 ± 0.1 mg/mL, resp. Concentrations normalized to 100% of product label were 74%, 97%, 100%, 104%, 109% and 102%, resp. E-liquid brand 1 showed significance (p < 0.001) between bottles, while the reference showed the least variability. Similar results were obtained for the NRAs. Results also indicated the NRAs in aerosol of the reference E-liquid are lower than in cigarette smoke. The amounts of NRAs present in E-liquids and E-liquid aerosol are less compared to cigarettes, however, inconsistencies and variation in nicotine concentrations supports the need for regulatory oversight. Here is a brief introduction to this compound(494-52-0)COA of Formula: C10H14N2, if you want to know about other compounds related to this compound(494-52-0), you can read my other articles.

Reference:
Metal catalyst and ligand design,
Ligand Template Strategies for Catalyst Encapsulation – NCBI